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. 2004 Jun;24(11):4757–4768. doi: 10.1128/MCB.24.11.4757-4768.2004

FIG. 2.

FIG. 2.

CM from Wnt5a-expressing cells induces Dvl phosphorylation. (A) Rat2 fibroblasts were treated for 2 h with CM from Rat2 cells expressing control vector, Wnt1, or Wnt5a. Extracts were then analyzed as described for Fig. 1A for phosphorylation of Dvl and cytosolic β-catenin. Analysis of cytosolic GSK3β protein levels provided a loading control. (B) Treatment of 10T1/2 fibroblasts with Wnt5a CM also induces Dvl phosphorylation. (C) Kinetics of Dvl phosphorylation induced by Wnt5a. 10T1/2 fibroblasts were treated with either control or Wnt5a CM for the indicated times. Cells were then lysed, and extracts were analyzed by Western blotting with Dvl antibodies as described for panels A and B.