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Lithium treatment stabilizes β-catenin but has no effect on Dvl phosphorylation. Rat2 cells were treated for 2 h with 40 mM KCl, 40 mM LiCl, control CM, or Wnt3a CM. Extracts were then analyzed as described for Fig. 1A for cytosolic β-catenin levels, Dvl phosphorylation, and cytosolic GSK3β as a loading control. The relative increases in β-catenin levels determined by densitometry are indicated.
