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MLC2V-Hand1/eHAND transgenic mice. (A) Schematic representation of the transgene. (B) The MLC2V promoter drives transgene expression in the RV and OFT but not in the boundary region. (C) Immunohistochemistry with an anti-FLAG antibody revealed FLAG-tagged Hand1/eHAND protein expression in the RV but not in the IVS. (D) In MLC2V-Hand1/eHAND transgenic embryos, the IVS formed normally. Av, AvaII; E, EcoRI. Bars, 100 μm.
