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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Immunity. 2013 Oct 24;39(5):963–975. doi: 10.1016/j.immuni.2013.10.005

Figure 3. The increased resistance of passively immunized C57BL/6 WT mice to challenge with a potentially lethal dose of BV is dependent on functional serum IgE.

Figure 3

(A) Experimental outline. Donor mice were injected with 2× 200 µg BV or PBS. Three weeks later, sera from PBS-or BV-injected mice ([PBS-serum] or [BV-serum], respectively) were collected (see also Figure S2), and pooled BV-serum was processed in vitro to neutralize IgE function, either by heating or anti-mouse IgE treatment ([heated BV-serum] or [anti-IgE BV-serum], respectively). Age-matched recipient mice were anesthetized and transfused i.v. with serum 22 h before challenge with 4× 200 µg BV. (B) Changes in body temperature after challenge (Δ Temp [mean ± SEM]) and (C) survival (% of live animals) were monitored at indicated time points. Data are pooled from experiments obtained with 2 different pools of serum and a total of 4 independent experiments (n=14–20/group). P values are calculated by (B) Student’s t test and (C) Mantel-Cox test. *, P<0.05; **, P < 0.01; ***, P < 0.001 for the indicated comparisons. See also Figure S3.