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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: Immunity. 2013 Oct 24;39(5):963–975. doi: 10.1016/j.immuni.2013.10.005

Figure 4. IgE, but not IgG antibodies, are necessary for the protective effect of the adaptive response to BV.

Figure 4

(A) Experimental outline for panels B and C. (B and C) IgE-deficient (Igh-7−/−) or IgE-sufficient (Igh-7+/+) BALB/c mice mice were injected with 1× 100 µg BV or PBS. Three weeks later, mice were challenged with 5× 200 µg BV. (B) Changes in body temperature (Δ Temp) and (C) survival (% of live animals) were monitored at the indicated times. (D) Experimental outline for panels E and F. WT BALB/c donor mice were injected with 1× 100 µg BV or PBS. Three weeks later, sera from PBS- or BV-injected mice ([PBS-serum] or [BV-serum], respectively) were collected, and pooled BV-serum was processed in vitro to neutralize IgE function by heating (heated BV serum). Age-matched IgE-deficient recipient mice were anesthetized and transfused i.v. with serum or PBS containing monoclonal anti-DNP-IgE (an amount equivalent to the total IgE content of transferred BV-serum) 22 h before challenge with 5× 200 µg BV. (E) Changes in body temperature after challenge (Δ Temp) and (F) survival (% of live animals) were monitored at the indicated times. Data are pooled from (B and C) 4 independent experiments (n=17–19/group) or (E and F) from experiments obtained with 2 different pools of serum and a total of 3 independent experiments (n=8–14/group). (B and E) Values are mean ± SEM. P values are calculated by (B and E) Student’s t test or (C and F) Mantel-Cox test. *, P < 0.05; **, P < 0.01; ***, P < 0.001 for the indicated comparisons; the numbers in B, C, E and F are the P values for indicated comparisons that were not significant (P > 0.05). See also Figure S4.