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Immunohistochemical analysis of ZO-1 in colonic mucosa from rats with PI-IBS. Original magnification 400x. Normal control rats (a), PI-IBS model rats (b), and TMT treated rats (c), low-dose of HXZQ rats (d) rats, middle-dose of HXZQ treated rats (e), and high-dose of HXZQ rats (f). Semiquantitative analysis of ZO-1-staine areas showed a significant decrease in rats with PI-IBS, and HXZQ could significantly increase these areas.
