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. 2004 Jun;24(11):4979–4993. doi: 10.1128/MCB.24.11.4979-4993.2004

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Copyright © 2004, American Society for Microbiology

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FIG.8. — The nucleocytoplasmic distribution of Abi-1 is regulated by the Wave-1- and Syntaxin-1-binding regions and the SH3 domain. (A) Cellular localization of the indicated Abi-1 mutants before (−) and after (+) LMB treatment. The number on the bottom right in each panel is the percentage of cells with Abi-1 nuclear staining. (B) Localization of the indicated EYFP-tagged Abi-1 mutants in NIH 3T3 cells in the absence of LMB. The number on the bottom right in each panel is the percentage of cells with Abi-1 nuclear staining. (C) Schematic representation of the Abi-1 mutants employed in this study; their localization is indicated. C, cytoplasmic localization; N, nuclear localization. The Abi-1 nomenclature is given in the legend to Fig. 2G.