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. 2014 Sep 15;9(9):e106568. doi: 10.1371/journal.pone.0106568

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© 2014 Ebrahim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Fourier transform infra-red (FTIR) spectra of HMW-bLf indicating characteristic peaks and compared with other forms of bLf. B) Differential scanning calorimetry thermograms of the different bLf forms C) SDS-PAGE showing the comparative resistance of HMW-bLf to Omnizyme (human digestive enzyme cocktail) treatment. It also gives an indication that HMW-bLf is an oligomer made of bLf monomers; because upon digestion HMW-bLf besides dimers and trimers does not release any other fragments lower than ∼78 kDa apart from the ones produced from the digestion of commercially pure NM-bLf.