Table 2. Consensus sequence and number of sequence variants found in ISR building blocks.
Building block | Sequence length | Nr. of sequences | Nr. of variants | Consensus sequence |
Start | 29 bp | 95 | 4 | AAGGAGAATTACCTACTGTTTAATTTTGA |
Ntrna | 26 bp | 72 | 5 | GGGTTCGTTTTTACGAATACTCAAAA |
Trna | 186 bp | 23 | 11 | AAGTTCTTTACGAACTTTATATATGGGGGTGTAGCTCAGTTGGGA GAGCACTTGCCTTGCAAGCAAGGGGTCAGGAGTTCGACTCTCCTC ATCTCCACCATTTAAGAGTATATTACTTAAATCTTTGATTTACTTAG TAGCCTCTTACAATGCACTTATAGCTTAAATTTATACAAGCTTTGTGCG |
DR | 9 bp | 335 | 8 | TTAGCACTT |
20 bp | 20 bp | 3 | 1 | TAAGCAACGGAATTTATTCG |
33 bp | 33 bp | 132 | 42 | TAAGCAACGGAATTTATTCGTTGGCGCTGTGCT |
53 bp | 53 bp | 98 | 35 | TAAGCAACAGAATAAACTGAACGCATGTGAAGTTTGTTTGTTG GCGCTGTGCG |
End | 114 bp | 95 | 33 | TGAAAACTGCATATATATATTTAGTGATATGACATCTAATTTGTA ATATATAAAGCTGATAACTTTTTAAAATTATCGAAGTTGATAGCT TCTAATCTATCAAACCTTTTTAAC |
Only the representatives (n = 95) of non-redundant sets were used to calculate the consensus sequence and to determine the number of sequence variants.