Study	Reason for exclusion
Abrao 2008	Not an RCT. In this case-control study, 108 women were treated with 5-day MTX (42 women) or 5-day DACT (42 women) or MTX/DACT combined (24 women). The combined intervention was stopped due to high rates of toxicity
Berkowitz 1979	Not an RCT. The study may have high-risk patients. It was not clear if patients were balanced for demographic variables It was not clear if all patients between 1976 to 1978 were treated with MTX @ 6mg/kg or if the decision to use this dose was left to attending physician
Gleeson 1993	Not an RCT. High risk of selection bias “ the choice of treatment was at the discretion of the attending oncologist” Follow-up period not clear.
Kohorn 1996	Not an RCT. In this case-control study, women were treated with a 5-day DACT regimen (43 women) or a pulsed DACT regimen (18 women)
Matsui 1998	High risk of selection bias; patients were not matched for the potential confounding variables in the different treatment groups Included in subsequent publication.
Matsui 2005	High risk of selection bias; study did not provide information about patient characteristics and if they were matched for the potential confounding variables in the different treatment groups
Petrilli 1980	High risk of selection bias: study did not provide information about the characteristics of patients and if they were matched for potential confounding variables in the treatment groups
Roberts 1996	Case series rather than case-control study; 61 patients received MTX, 4 ACT and 5 MACT Risk of selection bias; patients were not matched for the potential confounding variables
Smith 1982	Not an RCT. In this case-control study, 39 women received MTX and 29 women received MTX-FA
Wong 1985	Not an RCT. In this case-control study, 33 women received MTX and 68 women received MTX-FA

DACT = Dactinomycin or Actinomycin D; MTX = Methotrexate; MTX-FA = methotrexate-folinic acid; RCT = randomised controlled trial