Figure 3. Mean-squared displacement (MSD) of selected beads within each chain. Short-lag time behavior is sub-diffusive, while the plateau a large lag times comes from the confinement of the nucleus wall and tethering of the chains. The inverse of the plateau value gives an approximation of the range of motion of the beads.²⁶ Beads close to the tethers explore smaller spaces than beads in the middle of the chain.