Figure 4. Chromatin remodeling in HGPS fibroblasts. The experiment was performed using the histone deacetylase inhibitor trichostatin A or combination of trichostatin A and statins (that convert progerin into its non-farnesylated form and elicit accumulation of wild-type prelamin A). The images obtained using anti-trimethyl-H3K9 antibody to label heterochromatin domains have been elaborated using Photoshop 7. Note recovery of heterochromatin domains (gray) using combined drugs, indicating that heterochromatin status in HGPS is regulated by prelamin A forms, most likely through histone deacetylase interaction.