Figure 3. The chromatin picture induced by treatment of MCF-7 cells with RA essentially mirrors that observed with E2. (A) ChIP assays to assess the key nucleosomal modifications and transcription factors assembly to the paradigmatic RA-responsive cyp26A promoter. (B) Evaluation by ChIP of the effect of IKKα knock down on presence of H3S10 ph and 8-oxo-Gs near the same promoter. (C) PARP fragmentation (left), cell count (middle), and FACS analysis (right) to assess the role of the control on ROS production played by IKKα, whose derangement induced apoptosis in RA-treated cells. The reported fractions of apoptotic MCF-7 cells represent the mean from three different experiments.