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Figure 1. ChIP assay for the in vivo quantification of LRPPRC binding to the ABCB1 promoter. RT-qPCR quantification of LRPPRC binding to the ABCB1promoter in K562 and Lucena cells after ChIP assay. DNA amplification was quantified in bound and unbound fractions after normalization with SMAD8 nonspecific amplification. Normalized fractions were used to calculate the bound/input ratio. Results are expressed as mean ± SD for three independent experiments.
