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. 2014 Sep 15;9(9):e107247. doi: 10.1371/journal.pone.0107247

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© 2014 Fujiwara et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Mice were subcutaneously transplanted with parental, CD166+, and CD166- cells from the Panc-1 cell line (representative image), and tumor volumes were regularly measured for 7 weeks. (B) Mouse orthotopic tumor xenograft models were also generated from CD166+ and CD166- Panc-1 cells. Tumors were excised and wet weighed. (C, E) Immunohistochemical analysis of CD166 in subcutaneous (C) and orthotopic (E) tumors derived from CD166+ and CD166- SW1990 and Panc-1 cells. Original magnification: 100×. (D, F) CD166 expression was analyzed in subcutaneous (D) and orthotopic (F) tumors derived from CD166+ and CD166- cells by flow cytometry. (G) Analysis of the relationship between tumor weight and the positivity rate of CD166 cells in orthotopic tumors derived from CD166+ and CD166- Panc-1 cells. All graphs show the mean ± SD; *, p<0.05, **, p<0.01.