Figure 10. Summary of ORFeome construction and main results.

The dashed line denotes future directions for sugarcane ORFeome studies. This ORFeome can be used for gene discovery related to a range of traits since over 5,000 different categories (Table S4) have a full-length representative. Differentially expressed alleles in each sample and in the hybrid and their origin from each ancestral genotype can also be analyzed. Several types of polymorphisms and genetic variability can be further investigated. Both genome assembly and annotation can make use of this sugarcane ORFeome dataset to validate and improve results. TF, transcription factor; NAT, natural antisense transcript.