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(A) SHH mRNA expression levels at 10 days post-encapsulation (the level in 10-day 2D cultured cells were set as 1-fold; n = 3; ***, p < 0.001). Secretion of: (B) SHH and (C) VEGF from cells cultured on 2D TCP or 3D hydrogels at day 10 post-encapsulation (n = 3. *, **, *** = p < 0.05, 0.01, 0.001).
