Table 2. Infectivity of dengue viruses (DENVs) for mosquito (C6/36) and human (HuH7) cell lines.
Dengue virus | Titre of virus (log10TCID/ml) | ||||
Serotype | Strain | C6/36 without FBS | C6/36 with FBS | HuH7 without FBS | HuH7 with FBS |
DENV-1 | Hawaii | 6.5 | 5.5 | <1.0 | <1.0 |
31459 | 7.0 | 6.5 | <1.0 | 2.0 | |
31987 | 5.5 | 5.0 | <1.0 | 3.0 | |
32514 | 7.0 | 6.5 | 3.0 | 3.0 | |
62699 | 6.0 | 4.5 | <1.0 | <1.0 | |
63001 | 5.5 | 5.0 | <1.0 | 2.0 | |
75971 | 5.5 | 5.5 | <1.0 | 2.0 | |
84077 | 8.0 | 7.5 | 3.0 | 3.0 | |
84558 | 7.5 | 7.0 | <1.0 | 2.0 | |
I. C | 7.5 | 7.0 | <1.0 | 4.0 | |
DENV-2 | New Guinea C | 8.0 | 8.0 | 4.0 | 4.0 |
I. C | 8.0 | 8.0 | 4.0 | 4.0 | |
DENV-3 | H87 | 7.5 | 7.5 | <1.0 | 2.0 |
82899 | 7.0 | 5.5 | <1.0 | <1.0 | |
83468 | 5.0 | 4.5 | <1.0 | <1.0 | |
84014 | 7.0 | 6.5 | <1.0 | <1.0 | |
84700 | 4.5 | 3.5 | <1.0 | <1.0 | |
DENV-4 | H241 | 7.0 | 7.0 | <1.0 | 2.0 |
84711 | 6.5 | 5.5 | <1.0 | <1.0 | |
84087 | 7.0 | 6.5 | <1.0 | <1.0 |
DENVs isolated from clinical patients in C6/36 were infected in C6/36 and Huh7 with ten-fold dilutions to determine the relative titres in both cell types. Both cells (C6/36 and Huh7) were infected at the same time with the same dilution of DENVs to determine the relative titres.
I.C. Infectious clone derived DENV-2.