Figure 6. The xylose consumption phenotypes of the evolved Y127 and Y128 strains are dependent upon CpxylA and ScTAL1.

Extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured by YSI instrument and OD₆₀₀ readings, respectively, from cultures containing KanMX marker rescued versions of (A) GLBRCY127 (Y132) and GLBRCY132 xylAΔ or (B) Y132 and Y132 tal1Δ strains inoculated in aerobic YPX media. In (C), extracellular xylose concentrations (solid lines) and cell density (dashed lines) were measured as in (A, B) for KanMX marker rescued GLBRCY128 (Y133) and two independent GLBRCY133 xylAΔ strains inoculated in anaerobic YPX media. These selection marker-rescued Y128 strains were cultured in YPD media and total RNA isolated from a single time point. Expression of CpxylA was then quantified and normalized to ScERV25 RNA levels by qPCR. The bar graph in (D) displays the average values and standard deviations for CpxylA RNA from three independent biological replicates.