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. 2014 Sep 15;9(9):e107499. doi: 10.1371/journal.pone.0107499

Figure 7. Y128 has a mutation in GRE3 that reduces xylitol production and contributes towards anaerobic xylose fermentation.

Figure 7

Fermentation samples were taken at the indicated time points marked by vertical colored bars in Fig. 5B and C. Cells were then filter-captured, briefly washed and then intracellular metabolites extracted. Average concentrations of xylose (A), xylulose (B), xylulose-5-phosphate (C) and xylitol (D) from independent duplicate fermentations were determined by reverse phase ion pairing HPLC-ESI coupled with MS/MS or gas chromatography (see Materials and Methods). Average concentrations and standard deviations are based on two biological replicates. Y22-3, Y127 and Y128 strains with KanMX selection markers excised (Y36, Y132 and Y133, respectively) and with or without deletion of GRE3 were cultured under anaerobic conditions in YPX media. Samples were taken at the indicated time points to measure xylose concentrations (E) or cell density (F). Average values and standard deviations were calculated from biological triplicates.