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. 2014 Sep 15;9(9):e107678. doi: 10.1371/journal.pone.0107678

Figure 2. Salinity represses auxin signaling pathway.

Figure 2

Seven dpg TIR1pro:TIR1-GUS (A) and AFB2pro:AFB2-GUS (B) seedlings were incubated in liquid ATS medium with increasing concentrations of NaCl for 4 h and then subjected to GUS staining. Representative photographs of root tips are shown. The control value is arbitrarily set to 1 in each case. Data are mean values of three independent experiments. (C) Seven dpg tir1-1 35S::TIR1-Myc plants were transferred to liquid ATS medium supplemented with 200 mM NaCl for different times. Total proteins were extracted and protein blot analysis was carried out using an anti cMyc antibody (upper panel). Ponceau staining of Rubisco is shown (bottom panel). (D) Densitometric analysis of three independent immunoblots as indicated in (C). Data are mean values (±SE). Different letters indicate a significant difference at P≤0.05 (Tukey test). (E) Seven dpg HSpro:AXR3NT-GUS seedlings were transferred for 2 h to 37°C and then incubated with 100 and 200 mM NaCl in the absence (upper panel) or presence (lower panel) of IAA. GUS activity was revealed after incubation with X-Gluc at 37°C. Representative photographs and the GUS signal detected in NaCl treatment relative to control is shown. The control value is arbitrarily set to 1 in each case. Data are mean values of three independent experiments.