Figure 5. ROS accumulation in LR of mir393ab seedlings during acclimation to salinity.

Four dpg WT and mir393ab seedlings were transferred onto ATS medium containing 75 mM NaCl. After 5 d of treatment, endogenous H₂O₂ was detected in situ by H2DCF DA probe. Quantification of probe fluorescence intensity in LRs is shown. LR were selected from the same position on the primary root. a.u., arbitrary units. Data are mean values (±SE) of three independent experiments. Different letters indicate a significant difference at P≤0.05 (Tukey test).