Table 1. Primers used in this study.
| Primers | Sequence (5′–3′) | Usage |
| Bar5 | CGCCTGGACGACTAAACC | Confirmation of gene disruption |
| Bar3 | TCAGCCTGCCGGTACCGC | |
| Sur5 | ATCGTGGAGTCATGTTTG | Confirmation of gene disruption |
| Sur3 | CCAGTAAGTAATATATCC | |
| DMrKu70-51(B2r+5F) | ggggacagctttcttgtacaaagtggaaAGCCAGGTCCCTTATCCC | Disruption of MrKu70 |
| DMrKu70-52(B1r+5R) | ggggactgcttttttgtacaaacttgtCCGAGTAGAAACAATTC | |
| DMrKu70-31(B4+3F) | ggggacaactttgtatagaaaagttgttGACTGCTTTCATTGGTG | |
| DMrKu70-32(B3+3R) | ggggacaactttgtataataaagttgtTGTGCCAATTTGGCAGCC | |
| MrKu70-CF1 | GAATAGAGCAATGGATAG | Confirmation of disruption of MrKu70 |
| MrKu70-CF2 | CCCTTTAGACTCGCCATC | |
| MrKu70-5 | CCCGGGGCGTACAAGTGATGCTAC | Complementation of ΔMrKu70 |
| MrKu70-3 | CCCGGGAAGTGACGATTCAGATCC | |
| MrKu70ORF-5 | ATGGGGATCAAGAGATTG | Complementation of ΔMrKu70 |
| MrKu70ORF-3 | GATACACCACGCAATGCC | |
| DCag8-51(B2r+5F) | ggggacagctttcttgtacaaagtggaaAGCAATTGTATTTGCTGG | Disruption of Cag8 |
| DCag8-52(B1r+5R) | ggggactgcttttttgtacaaacttgt ACGGAGTCAAGTATGGAG | |
| DCag8-31(B4+3F) | ggggacaactttgtatagaaaagttgttGGTAAATACCAGCAAGTG | |
| DCag8-32(B3+3R) | ggggacaactttgtataataaagttgtTTTCTTATTTGCGGTTGG | |
| Cag8-CF1 | TGTTACCACGACGACAAC | Confirmation of disruption of Cag8 |
| Cag8-CF2 | TTTACTGACGTGACGGTG |
Note:
B2r+5F: the Bp Clonases recognition site B2r (lowercase) and the forward primer 5F (upper case) to amplify the 5′ flanking sequences.
B1r+5R: the Bp Clonases recognition site B1r (lowercase) and the reverse primer 5R (upper case) to amplify the 5′ flanking sequences.
B4+3F: the Bp Clonases recognition site B4 (lowercase) and the forward primer 3F (upper case) to amplify the 3′ flanking sequences.
B3+3R: the Bp Clonases recognition site B3 (lowercase) and the reverse primer 3R (upper case) to amplify the 3′ flanking sequences.