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. 2014 Sep 15;9(9):e107657. doi: 10.1371/journal.pone.0107657

Table 1. Primers used in this study.

Primers Sequence (5′–3′) Usage
Bar5 CGCCTGGACGACTAAACC Confirmation of gene disruption
Bar3 TCAGCCTGCCGGTACCGC
Sur5 ATCGTGGAGTCATGTTTG Confirmation of gene disruption
Sur3 CCAGTAAGTAATATATCC
DMrKu70-51(B2r+5F) ggggacagctttcttgtacaaagtggaaAGCCAGGTCCCTTATCCC Disruption of MrKu70
DMrKu70-52(B1r+5R) ggggactgcttttttgtacaaacttgtCCGAGTAGAAACAATTC
DMrKu70-31(B4+3F) ggggacaactttgtatagaaaagttgttGACTGCTTTCATTGGTG
DMrKu70-32(B3+3R) ggggacaactttgtataataaagttgtTGTGCCAATTTGGCAGCC
MrKu70-CF1 GAATAGAGCAATGGATAG Confirmation of disruption of MrKu70
MrKu70-CF2 CCCTTTAGACTCGCCATC
MrKu70-5 CCCGGGGCGTACAAGTGATGCTAC Complementation of ΔMrKu70
MrKu70-3 CCCGGGAAGTGACGATTCAGATCC
MrKu70ORF-5 ATGGGGATCAAGAGATTG Complementation of ΔMrKu70
MrKu70ORF-3 GATACACCACGCAATGCC
DCag8-51(B2r+5F) ggggacagctttcttgtacaaagtggaaAGCAATTGTATTTGCTGG Disruption of Cag8
DCag8-52(B1r+5R) ggggactgcttttttgtacaaacttgt ACGGAGTCAAGTATGGAG
DCag8-31(B4+3F) ggggacaactttgtatagaaaagttgttGGTAAATACCAGCAAGTG
DCag8-32(B3+3R) ggggacaactttgtataataaagttgtTTTCTTATTTGCGGTTGG
Cag8-CF1 TGTTACCACGACGACAAC Confirmation of disruption of Cag8
Cag8-CF2 TTTACTGACGTGACGGTG

Note:

B2r+5F: the Bp Clonases recognition site B2r (lowercase) and the forward primer 5F (upper case) to amplify the 5′ flanking sequences.

B1r+5R: the Bp Clonases recognition site B1r (lowercase) and the reverse primer 5R (upper case) to amplify the 5′ flanking sequences.

B4+3F: the Bp Clonases recognition site B4 (lowercase) and the forward primer 3F (upper case) to amplify the 3′ flanking sequences.

B3+3R: the Bp Clonases recognition site B3 (lowercase) and the reverse primer 3R (upper case) to amplify the 3′ flanking sequences.