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. 2014 Sep 6;3:502. doi: 10.1186/2193-1801-3-502

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© Kumar et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Effects of ARE-BPs on expression from luciferase reporter containing BDNF-L or BDNF-S. a. Effects of TTP, BRF1, BRF2, ELAVL1, ELAVL2 and AUF1 on the expression of luciferase reporter containing BDNF-L (Luc-BDNF-L) compared to Green Fluorescent Protein (GFP) and empty vector (pDEST40) as negative controls in HEK-293 cells, N = 2-8 experiments with 3–4 repeats per experiment, ***p < 0.001, #p < 0.035, Error bars indicate SD. b. Comparison of effects of TTP, BRF1 and BRF2 on Luc-BDNF-L and Luc-BDNF-S expression in HEK-293 cells revealed no statistically significant difference between BDNF-L and BDNF-S, N = 3-4, experiments with 4 repeats per experiment, ***p < 0.001. Error bars indicate SD. c. Effect of TTP expression on Luc-BDNF-S mRNA turn over in the HEK-293 cells upon Actinomycin D (1 μg/ml) treatment at the indicated time points, N = 2 experiments, a representative experiment is shown. BDNF-S and β-actin mRNA was detected with semi-quantitative PCR.