FIG. 6.

NKAα3 is colocalized with glutamate receptors and transporters. High-magnification micrographs of the striolar region of the utricle double immunolabeled with a mouse monoclonal anti-NKAα3 (green, A and D) and either rabbit polyclonal anti-GluA2/3 (red, B) or goat polyclonal anti-EAAT5 (red, E) reveal NKAα3 immunoreactivity colocalized with GluA2/3-positive puncta (GluA2/3) on the calyx inner face (C) and EAAT5 immunoreactivity present on both the calyceal inner face and also the outer membrane (F). Similar patterns of immunoreactivity were observed in high magnification views of the anterior and horizontal cristae (data not shown). Micrographs represent single optical sections to better examine colocalization. Where clearly resolved, the inner (open arrowhead) and outer (closed arrowhead) calyx membranes are indicated (A, D). Scale bars measure 10 μm.