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. 2014 Aug 30;14:640. doi: 10.1186/1471-2407-14-640

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© Tobar et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TGF-β1 increased intracellular ROS production and CTGF, NOX-4 and α-SMA mRNA expression. Time-course evaluation of CTGF, NOX-4 and α-SMA mRNA expression performed by qPCR. RMF-EG cells cultures, previously deprived of serum were stimulated with 5 ng/ml of TGF-β1 and their mRNA levels were evaluated at the indicated times. In a parallel group of cells, intracellular ROS production was assessed by measuring fluorescence generated after H₂DCFDA incubation. Figure is representative of three independent experiments. Values were normalized by number of viable cells at the end of the experiment.