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. 2014 Aug 30;14:640. doi: 10.1186/1471-2407-14-640

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© Tobar et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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NADPH oxidase activity modulated the expression of CTGF and α-SMA. A. Inhibition of NOX activity decrease CTGF and α-SMA expression. Time-course expression of CTGF and α-SMA mRNA evaluated by qPCR in 5 ng/ml TGF-β1- stimulated RMF-EG cells pre-treated or not with DPI. B. DPI inhibited TGF-β1-induced CTGF and α-SMA protein expression. RMF-EG cells were treated with TGF-β1 (5 ng/ml) in the presence or absence of 2,5 μM DPI. Protein expression of CTGF and α-SMA was evaluated by western blot after 24 h and 72 h, respectively. C. Densitometric analysis of western blots bands in B. Columns represent the mean of α-SMA to Tubulin signal and CTGF to Actin signal from three independent experiments; bars ± SE D. DPI inhibits TGF-β1-induced 3TP-luc luciferase reporter activity. RMF-EG cells transiently transfected with 3TP-luc luciferase construct and were pre-treated or not with DPI before stimulation with TGF-β1 for 16 h. Values are average of three separate experiments performed in triplicate.