Figure 2. STV11 haplotypes and complementation tests.

(a) STV11 haplotypes identified in 24 rice varieties. AA changes are shown above the closed bar. *indicates non-synonymous polymorphisms. In, indica; Jp, japonica. (b–g) Acquired RSV resistance of transgenic lines overexpressing STV11-R driven by the maize Ubiquitin-1 promoter (b–d) or transformed with a genomic sequence of STV11-R (e–g). NIP, the susceptible wild type NIP. (c,f) Quantification of RSV resistance. (d,g) Measurement of RSV by ELISA assay. One-week-old plants were used for RSV infection. Photographing, resistant evaluation and ELISA were performed 30 (b–d) or 70 (e–g) d.p.i. Data are shown as means±s.e.m. (n=3). Different letters at the top of each column indicate a significant difference at P<0.01 (n=3) by the Student’s t-test.