Table 1. Rates of co-translocational unfolding and translocation.
| Trx construct | Rate step 2→3 | Rate step 3→4 | Rate step 4→1 |
|---|---|---|---|
| oligo(dC)30-S1C-V5, +100 mV | 140±10 s−1 | 0.47±0.03 s−10.0058±0.0002, s−1 | 220±20 s−1 |
| oligo(dC)30-S1C-V5, +140 mV | 690±10 s−1 | 0.47±0.2 s−10.0087±0.0003, s−1 | 120±20 s−1 |
| V5-C109-oligo(dC)30, +100 mV | 6±1 s−1 | 1.5±0.1 s−1 | 100±30 s−1 |
| V5-C109-oligo(dC)30, +140 mV | 70±20 s−1 | 1.7±0.1 s−1 | 80±20 s−1 |
| oligo(dC)30-S1C-V5, ∇6G, +140 mV | Not detected | 0.62±0.05 s−10.0075±0.0005, s−1 | 210±10 s−1 |
| oligo(dC)30-S1C-V5, P22A-V23I, +140 mV | 800±70 s−1 | 0.8±0.2 s−10.0084±0.0004, s−1 | 190±20 s−1 |
| oligo(dC)30-S1C-V5, 2 M urea, +140 mV | 1,000±100 s−1 | 7±1 s−10.051±0.003 s−1 | 200±10 s−1 |
Trx, thioredoxin.
Values for Trx oligo(dC)30-S1C-V5 (N terminus first) were derived from exponential or biexponential fits to dwell-time histograms, in which the data from at least three independent experiments were compiled. Errors for N terminus-first pulling represent the coefficient confidence bounds (1σ) obtained in the fits. Values for Trx V5-C109-oligo(dC)30 (C terminus first) were obtained from ref. 11. Errors represent the s.d. from independent experiments (n=6).