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. 2014 Sep 5;5:4830. doi: 10.1038/ncomms5830

Figure 4. Sequential induction mediated gradually by FGF/MAPK/ERK on PCPs.

Figure 4

Activation of ERK1/2 visualized by dpERK1/2 antibody staining. Ciona embryos were electroporated with ptyrp1/2a>2xGFP and stained using anti-GFP antibody (green) to label the PCPs from early neurula (a) (a10.97s and a10.98s), late neurula (b,c) (a10.97s and a10.98s in division) to early tailbud stage (d) (a11.194s, a11.195s and a11.196s). Note that at early and late neurula stages, nuclear dpERK is detected in the a10.97s and their progenies but not in a10.98s. (a,b,d) Scale bar, 50 μm; (c) scale bar, 20 μm; nuclear staining is visualized by DAPI (blue). White asterisks and arrowhead indicate a10.98s and a10.97s and their cell progenies, respectively. Number of embryos showing dpERK1/2 staining out of the total embryos scored. Each experiment was repeated at least three times.