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. 2014 Sep 5;5:4830. doi: 10.1038/ncomms5830

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Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

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Expression pattern at tailbud stage of PCP candidate genes (red): Ci-bzrap,(a), Ci-Slc45a2 (b), Ci-Lrp4 (c), Ci-Mnt (d), Ci-Casz1(e), Ci-Piwi (f) and Ci-doc2a (g); co-localization (merge) of these PCP markers with Ci-tyrp1/2a (green),higher magnification on the right. Schematic representation of a9.49-derived cells in the neural tube of Ciona tailbud stage showing Ci-tyrp1/2a⁺ cells (green) and PCP candidates expressing cells (red), adapted from Cole and Meinertzhagen, 2004.tyrp1/2a transcripts are accumulated mostly in a10.97 progeny (a11.193s/a11.94s, green) than a10.98s derivatives (a11.195s/a11.96s, light green) that maintains only a residual amount of tyrp1/2a RNAs. Lateral view, anterior is on the left. Scale bar, 50 μm; scale bar, 20 μm for zoomed embryo; nuclear staining by DAPI (blue). Number of embryos scored was higher than 30; each experiment was repeated at least two times.

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