Figure 3. Point mutations in OSM-9 alter Ca²⁺ transients in ASH.

(a,b) Heat maps for osm-9::gfp WT rescue and osm-9 (ky10) worms, depicting all worms tested for each genotype. Trial number is on the left axis and pseudo colour index is shown on the right axis. (c–f) Average Ca²⁺ transients in ASH neurons of osm-9(ky10) worms transiently expressing WT and mutant OSM-9::GFP. Averages are generated from 12 to 16 animals for each strain, where each animal is stimulated and analysed once. All transients are displayed as ΔF/F of G-CaMP (see Methods section). (a) osm-9::gfp WT rescue strain displays a WT Ca²⁺ transient. (c,d) Y604G and Y604F Ca²⁺ transients are flat and resemble that of osm-9(ky10) animals. (e) Y606A mutants display reduced Ca²⁺ transients in those animals that respond to stimuli, inset displays 10-fold reduced scale and resulting Ca²⁺ transient. (f) E607A mutant animals display altered Ca²⁺ dynamics. Raw data are displayed, so as not to lose inconsistencies in the individual trials.