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. Author manuscript; available in PMC: 2014 Sep 16.

Published in final edited form as: Leuk Res. 2010 Feb 19;34(7):925–931. doi: 10.1016/j.leukres.2010.01.020

Purified B-lymphocytes (1 × 10⁶cells/ml) from B-CLL patients were incubated in the presence of indicated concentrations of arsenic trioxide (Panel A) or ascorbic acid (Panel B) for indicated time periods. The cells were stained with Annexin-V-FITC and propidium iodide as described by us previously²³. The cells were analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=10)

Purified B-lymphocytes (1 × 10⁶cells/ml) from B-CLL patients were incubated in the presence of indicated concentrations of arsenic trioxide (Panel A) or ascorbic acid (Panel B) for indicated time periods. The cells were stained with Annexin-V-FITC and propidium iodide as described by us previously²³. The cells were analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=10)