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. Author manuscript; available in PMC: 2014 Sep 16.

Published in final edited form as: Leuk Res. 2010 Feb 19;34(7):925–931. doi: 10.1016/j.leukres.2010.01.020

Panel a: Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with either arsenic trioxide [ATO] (1μM), ascorbic acid (1mM), or with both agents together for the indicated time periods. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=12)

Panel b: Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with 0.5, 1 and 2μM arsenic trioxide [ATO] in conjunction with indicated concentrations of ascorbic acid for 24 hours. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=12; p<0.001).

Panel a: Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with either arsenic trioxide [ATO] (1μM), ascorbic acid (1mM), or with both agents together for the indicated time periods. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=12)

Panel b: Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with 0.5, 1 and 2μM arsenic trioxide [ATO] in conjunction with indicated concentrations of ascorbic acid for 24 hours. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry and data collected under list mode. The data shown represent % Annexin-V^-/PI^- viable cells ± SD that are normalized to media control. (n=12; p<0.001).