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. Author manuscript; available in PMC: 2014 Sep 16.

Published in final edited form as: Leuk Res. 2010 Feb 19;34(7):925–931. doi: 10.1016/j.leukres.2010.01.020

Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with 1μM arsenic and 1mM ascorbic acid either alone or in combination in the presence or absence of z-VAD-fmk (150uM) for 24 hours. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry for apoptotic cells (panel a). The data shown represent % Annexin-V⁺/PI⁺ cells ± SD.(n=3). Panel B shows Westernblot analysis of the lysates from one of the above experiments was assessed for incleaved and cleaved PARP. Fludarabin is used as a control in these studies.

Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were incubated with 1μM arsenic and 1mM ascorbic acid either alone or in combination in the presence or absence of z-VAD-fmk (150uM) for 24 hours. The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry for apoptotic cells (panel a). The data shown represent % Annexin-V⁺/PI⁺ cells ± SD.(n=3). Panel B shows Westernblot analysis of the lysates from one of the above experiments was assessed for incleaved and cleaved PARP. Fludarabin is used as a control in these studies.