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. Author manuscript; available in PMC: 2014 Sep 16.
Published in final edited form as: Leuk Res. 2010 Feb 19;34(7):925–931. doi: 10.1016/j.leukres.2010.01.020

Figure 4. Accumulation of reactive oxygen species by arsenic trioxide and ascorbic acid.

Figure 4

Purified B-lymphocytes from CLL patients (1×106/ml media) (1×106/ml) were treated with 1μM arsenic and 1mM ascorbic acid either alone or in combination. Subsequently DHE was added to 10μM final concentration and the cells were incubated for an additional 60 minutes. The cells were analyzed by flow cytometry and data collected under list mode. A representative histogram showing the vehicle control and arsenic trioxide [ATO]/ascorbic acid treated group is shown in the top panel. The bottom panel shows %DHE positive cells in media control, ATO (0.1uM), ascorbic acid (1mM) and ATO/ascorbic acid treated groups.