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. Author manuscript; available in PMC: 2014 Sep 16.

Published in final edited form as: Leuk Res. 2010 Feb 19;34(7):925–931. doi: 10.1016/j.leukres.2010.01.020

Panel a: N-acetyl cysteine (NAC) or Glutathione (GSH) mediated abrogation arsenic trioxide/ascorbic acid induced cytotoxicity in primary CLL B cells. Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were pretreated with Glutathione [GSH (1mM)] or N-acetyl cysteine (NAC) for 30 minutes prior to addition of ATO (1μM) and ascorbic acid (1mM). The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry after 24 hours. The data shown represent % Annexin-V^-/PI^- viable cells + SD that are normalized to media control. (n=6; p<0.05).

Panel b: Depletion of GSH by buthionine sulfoximine (BSO) enhances the As₂O₃ and ascorbic acid mediated cytotoxicity. Purified CLL B cells (1×10⁶/ml) were grown in the presence of 200μM BSO for 24 hours to deplete GSH levels. The cells were analyzed by flow-cytometry 24 hours after addition of ATO/ascorbic acid as described above.

Panel a: N-acetyl cysteine (NAC) or Glutathione (GSH) mediated abrogation arsenic trioxide/ascorbic acid induced cytotoxicity in primary CLL B cells. Purified B-lymphocytes from CLL patients (1×10⁶/ml media) were pretreated with Glutathione [GSH (1mM)] or N-acetyl cysteine (NAC) for 30 minutes prior to addition of ATO (1μM) and ascorbic acid (1mM). The cells were stained with Annexin-V-FITC and propidium iodide and analyzed by flow cytometry after 24 hours. The data shown represent % Annexin-V^-/PI^- viable cells + SD that are normalized to media control. (n=6; p<0.05).

Panel b: Depletion of GSH by buthionine sulfoximine (BSO) enhances the As₂O₃ and ascorbic acid mediated cytotoxicity. Purified CLL B cells (1×10⁶/ml) were grown in the presence of 200μM BSO for 24 hours to deplete GSH levels. The cells were analyzed by flow-cytometry 24 hours after addition of ATO/ascorbic acid as described above.