Fig. 4.

Confirmation of the presence of pCMVFUT and pGAL-GFPBsd gene construct in transgenic pigs by Southern hybridization analysis. a DNA from three transgenic piglets and wild-type pig, digested with EcoRI, fractioned on an agarose gel and hybridized with transgen-specific probe (464 bp for pGAL-GFPBsd). Lanes 1–3: DNA from piglets TG628, TG629 and TG632, respectively; lane 4: control DNA (wild-type pig); lane 5: transgene fragment mixed with nontransgenic genomic DNA representing 16 copies. b DNA from four transgenic piglets and wild-type pig, digested with BamHI, fractioned on an agarose gel and hybridized with transgen-specific probe (144 bp for pCMVFUT). Lanes 1–4: DNA from piglets TG588, TG604, TG605 and TG632, respectively; lane 5: control DNA (wild-type pig); lane 6: transgene fragment mixed with nontransgenic genomic DNA representing four copies. Note that both probes gave positive signal in case of DNA from TG632 what indicates that piglet as double transgenic animal