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. 2014 Feb 20;62(5):411–422. doi: 10.1007/s00005-014-0280-3

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© The Author(s) 2014

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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Fig. 5 — Transgene expression analysis by flow cytometry. Fibroblast derived from the following animals were analyzed: non-transgenic pig, TG588 transgenic pig expressing α1,2-fucosyltransferase, TG628 transgenic pig expressing α-galactosidase and TG632 transgenic pig expressing both α1,2-fucosyltransferase and α-galactosidase labelled by BS-IB4 lectin (detects α-Gal epitope), conjugated with AlexaFluor 647. X axis shows the fluorescence intensity, Y axis shows the number of cells, which were not labelled (black line), labelled non-transgenic cells (red line), cells from transgenic animal TG588 (blue line), cells from transgenic animal TG628 (dark-green line) and cells from transgenic animal TG632 (light-green line). The analysis showed decreased level of α-Gal antigen in transgenic animals compared to non-transgenic animals. The fluorescence fell by 60 % for the pig with α1,2-fucosyltransferase (TG588), 58.9 % for the pig with α-galactosidase expression (TG628) and 66.9 % for the pig with both α1,2-fucosyltransferase and α-galactosidase (TG632)