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. 2014 Aug 15;25(9):487–496. doi: 10.1007/s00335-014-9539-6

Fig. 2.

Fig. 2

A schematic of the breeding scheme for the Harwell Ageing Mutagenesis screen. C57BL/6 J males are ENU treated and breed to C3H/HeH females to produce G1s. G1 males are further backcrossed to C3H/HeH. G2 females are mated with their G1 founder to allow the identification of recessive mutations in the G3 generation, which enter the phenotyping pipelines. Each individual G3 mouse will carry a range of ENU-induced mutations that will be homozygous, heterozygous or wild type for any given mutation. Identifying multiple mice with the same phenotype allows the identification of the causative mutation by mapping as the ENU-induced mutations will only be present in regions of the genome derived from the founder (G0) strain, in this case C57BL/6 J

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