Fig. 2.

Combined reverse microdialysis and silicone probe recordings in freely moving rats. (A) Schematic drawing (AP = −3.14 from bregma, Paxinos and Watson, 2007) showing the position of the four shanks of the silicone probe (orange) and the microdialysis probe (black) with its active membrane shown in red. The dashed area indicates the zone of diffusion of the drug around the dialysis probe (see text for additional details). (B) Top view of the implant while the rat is positioned in the stereotaxic frame under isoflurane anesthesia, just before the insertion of the microdialysis probe. (C) Picture of the full implant in a freely moving rat. (D) Raw traces from 8 channels of the silicone probe (D1) obtained from a freely moving rat implanted as shown in C. Autocorrelograms (color) and crosscorrelograms (black) of 3 isolated neurons are shown in D2. (E) Plot showing the characteristic signature of T-type Ca²⁺ channel-mediated bursts recorded from a VB TC neuron in a freely moving rat. (F) Effect of reverse microdialysis application of 300 μM TTA-P2 on burst (F1) and total firing (F2) rate of the 3 neurons isolated as in D1-D2. Shaded and white areas indicate period of non-REM sleep and wakefulness, respectively.