Figure 2. Genetic map and synteny plots for the four references SUP05 Caudovirales contigs M8F6_0 (A), C22_13 (B), K04_0 (C) and G10_6 (D) (highlighted in bold).
Viral hallmark genes are underlined and identified on plots (MCP: major capsid protein, Sc: scaffolding protein, H-T conn.: head-tail connector). Sequence similarities were deduced from a tBLASTx comparison. For clarity sake, several sequences including SUP05 viral contig M8F6_0, K04_0, and G10_6 are reverse-complemented (noted RC).
DOI: http://dx.doi.org/10.7554/eLife.03125.008
Figure 2—source data 1. Summary of best BLAST hit affiliation for the predicted genes of the five SUP05 reference viral contigs.
For each contig, taxonomic and functional affiliation are indicated with the group or category and the number of genes affiliated to this group. The category ‘virion formation’ includes all genes associated to the formation of the capsid and the genome encapsidation.
elife03125s002.xls (10.5KB, xls)
DOI: 10.7554/eLife.03125.009
Figure 2—figure supplement 1. Phylogenetic tree of SUP05 Podoviridae contigs, derived from major capsid protein sequences with PhyML (maximum-likelihood tree, LG model, CAT approximation of gamma parameter).
All SUP05 contigs affiliated to the Podoviridae and harboring the major capsid protein gene are included in the tree and highlighted in bold. The three SUP05 Podoviridae reference contigs (longer than 15 kb) are noted with a star. SH-like branch supports are indicated on the tree, and all branches with a support lower than 0.5 were collapsed.
Figure 2—figure supplement 2. Phylogenetic tree for the SUP05 Microviridae (major capsid protein).
Tree was computed with PhyML (maximum-likelihood tree, LG model, gamma parameter estimated with CAT approximation), and SH-like supports are indicated for each branch. All branches with support lower than 0.50 were collapsed. The tree is focused around the Gokushovirinae subfamily and includes the Pichovirinae subfamily as an outgroup. Aquatic Gokushovirinae are colored according to their type of sample, and Saanich Inlet sequences are highlighted in bold. Cultivated Gokushovirinae are noted in black and highlighted in bold, with the associated genus associated in italic. All the other sequences are non-cultivated and currently affiliated to ‘Unclassified Gokushovirinae’.
Figure 2—figure supplement 3. Genetic map and synteny plots for the SUP05 Microviridae reference.
Viral hallmark genes are labeled on the plot. Associated sequence ‘Marine Gokushovirus isolate SOG1-KC131024’ was sampled from Strait of Georgia (Labonté and Suttle 2013b), on which the Saanich Inlet fjord is opening.