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. 2014 Jul 22;155(10):3981–3995. doi: 10.1210/en.2014-1163

Figure 2.

Figure 2.

A–C, A study to assess whether ezrin is an integrated component of the apical and basal ES in adult rat testes. A, Co-IP using lysates of seminiferous tubules was performed using specific markers of basal ES/BTB, apical ES, actin regulatory, and cytoskeletal proteins to identify specific protein-protein interaction with ezrin. IgG, both heavy (H) and light (L) chains, served as the protein loading control. Seminiferous tubule lysate (ST; 30 μg protein) served as positive control. +, positive protein-protein interaction with ezrin; −, negative protein-protein interaction with ezrin. B and C, To further confirm data from the Co-IP experiment, dual-labeled immunofluorescence analysis was performed to assess colocalization of ezrin (red) with either apical ES (green) proteins (B) or basal ES/BTB (green) proteins (C). Scale bar, 30 μm, in panels B and C, which applies to other micrographs. Data are representative findings from three experiments.