Figure 2.

PTHrP is required for the impaired chondrocyte maturation observed with phosphate restriction. Toluidine blue staining (TB), in situ hybridization for collagen type II (Col II) and collagen type X (Col X), and immunohistochemistry for p-Erk1/2 (pERK) were performed on wild-type, PTHrP +/−, and PTHrP −/− d15.5pc mouse metatarsals cultured in control (1.25 mM sodium phosphate) media or low phosphate (0.05 mM sodium phosphate) media for 4 days. Data are representative of 3 metatarsal elements for each condition.