Skip to main content
. 2014 Jul 22;155(10):3732–3738. doi: 10.1210/en.2014-1223

Figure 4.

Figure 4.

Preserved ARC 5-HT2CR–POMC appetitive machinery. A–D, No age-related differences in ARC gene expression were evident in male Pomc mRNA (t3 = 1.3, P = .27) (A) or ht2cr mRNA (t3 = 1.2, P = .31) (C) or female Pomc mRNA (t3 = 1.3, P = .51) (B) or ht2cr mRNA (t3 = 1.2, P = .29) (panel D) between young (3–5 months) and middle-aged (12–14 months) mice. E, A representative image of a coronal POMC-EGFP mouse brain slice illustrating EGFP neurons (green) loaded with fura-2 (red) in the ARC. All fura-2–loaded EGFP neurons (yellow, denoted with white arrow) were measured for changes in intracellular calcium, an assay of neuronal activation. Scale bar, 50 μm. F, Representative trace of POMC-EGFP neuron increasing intracellular calcium concentrations shown by decreasing fluorescence (F) expressed as ΔF/F to 20μM WAY-161503. G, 20 μM of 5-HT2CR agonist WAY161503 activates a similar proportion of ARC POMC neurons in young (23 from 60 cells) vs middle-aged (50 from 127) male EGFP brain slices using multiphoton fluorescence imaging to monitor changes in intracellular calcium (X2 (1) = 0.0184, P = .892). Data are presented as mean ± SEM, where appropriate. Abbreviation: 3v, third ventricle.