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. 2014 Jul 24;155(10):4027–4042. doi: 10.1210/en.2014-1132

Figure 2.

Figure 2.

Genotyping of organs from adult female (A–C) or male (D–F) deletion mutants and littermate controls. A, B, D, and E, Genotyping of the pituitaries from these mice. A and D, Mice 5–8 carry the Cre-recombinase transgene as a band at 166 bp. T-cell receptor δ (TCR-Δ) is a housekeeping gene seen in both controls (mice 1–4) and deletion mutants at 200 bp. Also included is DNA from wild-type (wt) and Cre+ animals on the other side of the ladder. The gels in B and F detect the presence of floxed Lepr exon 17 alleles (Lepr-exon17/loxP/loxP) in both controls and deletion mutants at 249 bp. No wt allele is detected (180 bp) except in the wt control (wt) on the other side of the ladder. B and E also show the presence of truncated Lepr (with exon 17 deleted) as a 228-bp band (LeprΔΔ) in the pituitaries of the Cre+ male or female mice (mice 5–8). C and F, Extrapituitary organ genotyping with primers for Lepr-exon 17/loxP/loxP. In females (C), there is no evidence for the presence of LeprΔΔ outside the pituitary, in deletion mutant mice (5–8). In contrast, organ genotyping of males (F) show LeprΔΔ in the testes from all deletion mutants (mice 5–8, arrow) and in the intestine from mouse 5 (arrow).