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. 2014 Jul 24;155(10):4027–4042. doi: 10.1210/en.2014-1132

Figure 6.

Figure 6.

Serum and mRNA levels of pituitary hormones in mutant females. A–C, Mutant diestrous females have significantly lower serum LH (P = .01, n = 15) (A), FSH (P = .03, n = 18) (B), and GH (P = .049; females, n = 21) (C). There were no significant changes in serum PRL (D), TSH (E), ACTH (n = 9) (F), or LEP (n = 8) (G). qPCR assays showed a 51% reduction in Fsh mRNA levels (P = .01, n = 5) (H) and 25% reductions in inhibin/activin β A and β B mRNA levels (P = .04, n = 5) (K). A star indicates significantly different from controls. There were no changes in mRNA levels for Lh, Cga, or Gnrhr (H), Gh or Ghrhr (I), and Tshβ, Prl, or Pomc (J). For the qPCR, the relative expression values were determined by the QuantStudio 12K Flex Software version 1.0 using the δ-δ-CT method.