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. 2014 Jul 24;155(10):4027–4042. doi: 10.1210/en.2014-1132

Figure 7.

Figure 7.

Serum and mRNA levels of pituitary hormones in mutant males. Mutant males show no change in LH (n = 19) (A) or FSH (n = 16) (B). However, mutants do show significantly reduced levels of serum GH (P = .016, n = 23) (C), PRL (P = .017, n = 9) (D), and TSH (P = .015, n = 9) (E). There were no reductions in serum ACTH (F) or LEP (G). qPCR assays showed no changes in Lh or Gnrhr mRNA levels, but there were 18% reductions in Fsh (P = .02, n = 5) and Cga (P = .01, n = 5) mRNA levels (H). There were 30%–40% reductions in mRNA levels of Gh (P = .02, n = 5) and Ghrhr (P = .03, n = 5) (I). There was no significant reduction in mRNA levels of inhibin/α-subunits (J) and Tsh, Pomc, or Prl (K). For the qPCR, the relative expression values were determined by the QuantStudio 12K Flex Software version 1.0 using the δ-δ-CT method.