Table 1.
Antibodies
| Peptide/Protein Target | Antigen Sequence (If Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in, Monoclonal or Polyclonal | Dilution Used |
|---|---|---|---|---|---|
| LEPR | Amino acids 577–594 | LEPR-extracellular domain | Novus Biologicals, Inc | Rabbit, polyclonal | 1:10 000 |
| LEPRb | OBR13S | Alpha Diagnostics Int Services | Rabbit, polyclonal | 1:10 000 | |
| GH | Rat GH | A. F. Parlow, Hormone Distribution Program | Rabbit, polyclonal | 1:200 000 | |
| LH-β | β-Subunit | Bovine LH-β | J. G. Pierce | Rabbit, polyclonal | 1:200 000 |
| LH-β | β-Subunit | Human LH-β | A. F. Parlow, Hormone Distribution Program | Rabbit, polyclonal | 1:5000 |
| FSH-β | β-Subunit | Human FSH-β | A. F. Parlow, Hormone Distribution Program | Rabbit, polyclonal | 1:5 000 |
| ACTH | 17-39 ACTH | Anti-17-39 ACTH | Produced in laboratory | Rabbit, polyclonal | 1:40 000 |
| PRL | Anti-rPRL | A. F. Parlow, Hormone Distribution Program | Rabbit, polyclonal | 1:10 000 | |
| TSH | β-Subunit | Anti-rTSH-β | A. F. Parlow, Hormone Distribution Program | Rabbit, polyclonal | 1:30 000 |
| GnRHR | Amino acids 1–328 | Anti-GnRHR (Fl-328) | sc-13944; Santa Cruz Biotechnology, Inc | Rabbit, polyclonal | 1:300 |
| Anti-pSTAT3 | Anti-pSTAT3 | Cell Signaling | Rabbit, polyclonal | 1:10 000 |