Table 3.

Bone Strength Testing by Three-Point Bending of the Femoral Diaphysis at 8 and 17 Weeks of Age, Reference Point Indentation of the Tibia in Male TH and SWR Mice at 17 Weeks of Age, and Static and Dynamic Histomorphometry of Proximal Tibial Metaphysis in Male TH and SWR Mice at 8 and 17 Weeks of Age (Mean ± SD)

	8 Weeks of Age		17 Weeks of Age		Pstrain	Pstrain*age
	SWR	TH	SWR	TH
n	6–10	6–10	6–10	6–10
Femoral three-point bending
Ultimate force, N	13.64 (1.50)	13.52 (1.03)	15.94 (1.37)	17.84 (1.26)a	.0565	.0307
Stiffness, N/mm	42.30 (7.85)	45.53 (6.79)	67.19 (7.22)	69.23 (9.41)	NS	NS
Estimated elastic modulus, GPa	3.27 (0.79)	5.00 (1.20)*	8.46 (1.12)	7.41 (2.03)	NS	.0069
Reference probe indentation
First indentation distance, ID first, μm	NA	NA	50.23 (8.65)	67.30 (22.95)a	.0411	NA
Indentation distance increase, last-first, μm	NA	NA	13.87 (5.67)	14.03 (4.15)	NS	NA
Static and dynamic histomorphometry
MS/BS, %	8.89 (4.52)	18.76 (3.37)a	15.77 (6.89)	8.83 (7.43)	NS	.0026
MAR, mm/d	0.92 (0.13)	1.38 (0.24)a	0.68 (0.08)	0.48 (0.40)	<.001	.0045
BFR/BS, mm3/mm2 · y	0.086 (0.058)	0.259 (0.062)a	0.108 (0.050)	0.042 (0.031)a	.0292	<.001
N.Ob/T.Ar, per mm	2.084 (0.835)	5.008 (2.022)a	0.98 (0.615)	1.359 (1.185)	.0088	.0363
N.Oc/T.Ar, per mm	5.403 (1.212)	3.83 (0.743)a	3.528 (2.02)	1.987 (1.332)	.0196	NS
N.Ad/T.Ar, per mm	0.29 (0.71)	14.54 (5.18)a	1.40 (1.04)	9.96 (6.64)	<.001	NS
BS, mm	14.45 (2.38)	7.62 (3.29)a	4.46 (1.76)	2.36 (1.22)a	<.001	.0016

Abbreviation: NA, data not available; N.Ad/T.Ar, number of adipocytes per total area; ID, indentation distance; MS, mineralizing surface; N.Ob/T.Ar, number of osteoblasts per total area; N.Oc/T.Ar, number of osteoclasts per total area.

^aBold text indicates significant difference from SWR (P < 0.05, two way ANOVA and Fisher's PLSD).